Simple jQuery Dropdowns
Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/1023
Title: Cytotoxicity evaluation of Clinacanthus nutans through dimethylthiazol diphenyltetrazolium bromide and neutral red uptake assays.
Authors: Suwanna Korsuwannawong
สุวรรณา ก่อสุวรรณวงศ์
La‑Ongthong Vajrabhaya
ละอองทอง วัชราภัย
Mahidol University. Faculty of Dentistry. Research Office
Suwanna Korsuwannawong
Keywords: Clinacanthus nutans;Dimethylthiazol diphenyltetrazolium bromide;Intra-class correlation coefficient;Neutral red uptake;Open Access article
Issue Date: Jan-2016
Citation: Vajrabhaya LO, Korsuwannawong S. Cytotoxicity evaluation of Clinacanthus nutans through dimethylthiazol diphenyltetrazolium bromide and neutral red uptake assays. Eur J Dent. 2016 Jan-Mar;10(1):134-8.
Abstract: Objectives: The aim of this study was to compare the results of dimethylthiazol diphenyltetrazolium bromide (MTT) and neutral red uptake (NRU) assays of Clinacanthus nutans cytotoxicity. Materials and Methods: Mouse fibroblast (L929) cells were exposed to 0.01%, 0.1%, 0.25%, and 0.5% (W/V) C. nutans in a 96-cluster -well-culture plate for 24 h. The cell viability after exposure to C. nutans was determined by MTT and NRU assays in separate tissue culture plates. The two assays were compared through an intra‑class correlation coefficient (ICC) analysis. Results: No significant differences in cytotoxicity were noted between the two assays ( P > 0.05). The ICC values for agreement between two assays for the negative and positive control groups and C. nutans concentrations of 0.01%, 0.1%, 0.25%, and 0.5% were 0.84, 0.83, 0.77, 0.68, 0.74, and 0.71, respectively. Conclusion: In general, the MTT and NRU assays performed similarly, exhibiting moderate to good correlation for the evaluation of the cytotoxicity of C. nutans .
URI: http://repository.li.mahidol.ac.th/dspace/handle/123456789/1023
metadata.dc.identifier.url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4784143/pdf/EJD-10-134.pdf
ISSN: 1305-7456 (printed)
1305-7464 (electronic)
Appears in Collections:DT-Article

Files in This Item:
File Description SizeFormat 
dt-ar-suwanna-2016-1.pdf1.33 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.