Simple jQuery Dropdowns
Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/11219
Title: Diversity of 16s-23s rDNA internal transcribed spacer (its) reveals phylogenetic relationships in burkholderia pseudomallei and its near-neighbors
Authors: Andrew P. Liguori
Stephanie D. Warrington
Jennifer L. Ginther
Talima Pearson
Jolene Bowers
Mindy B. Glass
Mark Mayo
Vanaporn Wuthiekanun
David Engelthaler
Sharon J. Peacock
Bart J. Currie
David M. Wagner
Paul Keim
Apichai Tuanyok
Northern Arizona University
Translational Genomics Research Institute
National Center for Emerging and Zoonotic Infectious Diseases
Menzies School of Health Research
Mahidol University
Keywords: Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology;Medicine
Issue Date: 14-Dec-2011
Citation: PLoS ONE. Vol.6, No.12 (2011)
Abstract: Length polymorphisms within the 16S-23S ribosomal DNA internal transcribed spacer (ITS) have been described as stable genetic markers for studying bacterial phylogenetics. In this study, we used these genetic markers to investigate phylogenetic relationships in Burkholderia pseudomallei and its near-relative species. B. pseudomallei is known as one of the most genetically recombined bacterial species. In silico analysis of multiple B. pseudomallei genomes revealed approximately four homologous rRNA operons and ITS length polymorphisms therein. We characterized ITS distribution using PCR and analyzed via a high-throughput capillary electrophoresis in 1,191 B. pseudomallei strains. Three major ITS types were identified, two of which were commonly found in most B. pseudomallei strains from the endemic areas, whereas the third one was significantly correlated with worldwide sporadic strains. Interestingly, mixtures of the two common ITS types were observed within the same strains, and at a greater incidence in Thailand than Australia suggesting that genetic recombination causes the ITS variation within species, with greater recombination frequency in Thailand. In addition, the B. mallei ITS type was common to B. pseudomallei, providing further support that B. mallei is a clone of B. pseudomallei. Other B. pseudomallei near-neighbors possessed unique and monomorphic ITS types. Our data shed light on evolutionary patterns of B. pseudomallei and its near relative species. © 2011 Liguori et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=83355170739&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/11219
ISSN: 19326203
Appears in Collections:Scopus 2011-2015

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.