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dc.contributor.authorSukanya Suttisrisungen_US
dc.contributor.authorSaengchan Senapinen_US
dc.contributor.authorBoonsirm Withyachumnarnkulen_US
dc.contributor.authorKanokpan Wongpraserten_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherThailand National Center for Genetic Engineering and Biotechnologyen_US
dc.date.accessioned2018-05-03T07:55:07Z-
dc.date.available2018-05-03T07:55:07Z-
dc.date.issued2011-12-01en_US
dc.identifier.citationJournal of Biosciences. Vol.36, No.5 (2011), 833-843en_US
dc.identifier.issn09737138en_US
dc.identifier.issn02505991en_US
dc.identifier.other2-s2.0-84858720130en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84858720130&origin=inwarden_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/11229-
dc.description.abstractA legume-type lectin (L-lectin) gene of the red algae Gracilaria fisheri (GFL) was cloned by rapid amplification of cDNA ends (RACE). The full-length cDNA of GFL was 1714 bp and contained a 1542 bp open reading frame encoding 513 amino acids with a predicted molecular mass of 56.5 kDa. Analysis of the putative amino acid sequence with NCBI-BLAST revealed a high homology (30-68%) with legume-type lectins (L-lectin) from Griffithsia japonica, Clavispora lusitaniae, Acyrthosiphon pisum, Tetraodon nigroviridis and Xenopus tropicalis. Phylogenetic relationship analysis showed the highest sequence identity to a glycoprotein of the red algae Griffithsia japonica (68%) (GenBank number AAM93989). Conserved Domain Database analysis detected an N-terminal carbohydrate recognition domain (CRD), the characteristic of L-lectins, which contained two sugar binding sites and a metal binding site. The secondary structure prediction of GFL showed a β-sheet structure, connected with turn and coil. The most abundant structural element of GFL was the random coil, while the α-helixes were distributed at the N- and C-termini, and 21 β-sheets were distributed in the CRD. Computer analysis of three-dimensional structure showed a common feature of L-lectins of GFL, which included an overall globular shape that was composed of a β-sandwich of two anti-parallel β-sheets, monosaccharide binding sites, were on the top of the structure and in proximity with a metal binding site. Northern blot analysis using a DIG-labelled probe derived from a partial GFL sequence revealed a hybridization signal of ∼1.7 kb consistent with the length of the full-length GFL cDNA identified by RACE. No detectable band was observed from control total RNA extracted from filamentous green algae. © Indian Academy of Sciences.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84858720130&origin=inwarden_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleIdentification and characterization of a novel legume-like lectin cDNA sequence from the red marine algae Gracilaria fisherien_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1007/s12038-011-9144-8en_US
Appears in Collections:Scopus 2011-2015

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