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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/11290
Title: Development of primers and a procedure for specific identification of the diatom Thalassiosira weissflogii
Authors: Saengchan Senapin
Kornsunee Phiwsaiya
Pauline Kiatmetha
Boonsirm Withyachumnarnkul
Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp)
Thailand National Center for Genetic Engineering and Biotechnology
Prince of Songkla University
Shrimp Genetic Improvement Center
Mahidol University
Keywords: Agricultural and Biological Sciences
Issue Date: 1-Aug-2011
Citation: Aquaculture International. Vol.19, No.4 (2011), 693-704
Abstract: A recent study showed Thalassiosira weissflogii to be a diatom containing suitable nutrition for larviculture of the black tiger shrimp, Penaeus monodon. Accurate and practical identification of this diatom species is therefore important for commercial hatcheries. The purpose of this study was to establish a DNA-based method of identification to supplement morphological examination, avoiding confusion with other Thalassiosira sp. Primers, 18SF/28SR1, specific for ribosomal DNA genes (3′-end of 18S rDNA through 5′-end of 28S rDNA, covering two internal transcribed spacers), were employed as a first-step polymerase chain reaction, followed by a second nested amplification using specifically designed primers, ITS1-F-D/ITS1-R-D. The nested-PCR result revealed specificity in the detection, distinguishing T. weissflogii from T. pseudonana, Cyclotella meneghiniana, and Chaetoceros sp., and the PCR fragment of the amplified region had a sequence that was 99% identical to the T. weissflogii sequence held by GenBank. © 2010 Springer Science+Business Media B.V.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79960074618&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/11290
ISSN: 1573143X
09676120
Appears in Collections:Scopus 2011-2015

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