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|Title:||Shrimp laminin receptor binds with capsid proteins of two additional shrimp RNA viruses YHV and IMNV|
Chu Fang Lo
Prince of Songkla University
Thailand National Center for Genetic Engineering and Biotechnology
National Taiwan University
|Keywords:||Agricultural and Biological Sciences;Environmental Science|
|Citation:||Fish and Shellfish Immunology. Vol.31, No.1 (2011), 66-72|
|Abstract:||Laminin receptor (Lamr) in shrimp was previously proposed to be a potential receptor protein for Taura syndrome virus (TSV) based on yeast two-hybrid assays. Since shrimp Lamr bound to the VP1 capsid protein of TSV, we were interested to know whether capsid/envelope proteins from other shrimp viruses would also bind to Lamr. Thus, capsid/envelope encoding genes from 5 additional shrimp viruses were examined. These were Penaeus stylirostris densovirus (PstDNV), white spot syndrome virus (WSSV), infectious myonecrosis virus (IMNV), Macrobrachium rosenbergii nodavirus (MrNV), and yellow head virus (YHV). Protein interaction analysis using yeast two-hybrid assay revealed that Lamr specifically interacted with capsid/envelope proteins of RNA viruses IMNV and YHV but not MrNV and not with the capsid/envelope proteins of DNA viruses PstDNV and WSSV. In vitro pull-down assay also confirmed the interaction between Lamr and YHV gp116 envelope protein, and injection of recombinant Lamr (rLamr) protein produced in yeast cells protected shrimp against YHV in laboratory challenge tests. © 2011 Elsevier Ltd.|
|Appears in Collections:||Scopus 2011-2015|
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