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|dc.contributor.author||Thomas D. Sullivan||en_US|
|dc.contributor.other||Faculty of Medicine, Thammasat University||en_US|
|dc.contributor.other||Thailand National Center for Genetic Engineering and Biotechnology||en_US|
|dc.contributor.other||Bowling Green State University||en_US|
|dc.contributor.other||University of Wisconsin School of Medicine and Public Health||en_US|
|dc.identifier.citation||Fungal Biology. Vol.115, No.7 (2011), 683-696||en_US|
|dc.description.abstract||Oomycetes are unique eukaryotic microorganisms that share a mycelial morphology with fungi. Many oomycetes are pathogenic to plants, and a more limited number are pathogenic to animals. Pythium insidiosum is the only oomycete that is capable of infecting both humans and animals, and causes a life-threatening infectious disease, called "pythiosis" In the majority of pythiosis patients life-long handicaps result from the inevitable radical excision of infected organs, and many die from advanced infection. Better understanding P. insidiosum pathogenesis at molecular levels could lead to new forms of treatment. Genetic and genomic information is lacking for P. insidiosum, so we have undertaken an expressed sequence tag (EST) study, and report on the first dataset of 486 ESTs, assembled into 217 unigenes. Of these, 144 had significant sequence similarity with known genes, including 47 with ribosomal protein homology. Potential virulence factors included genes involved in antioxidation, thermal adaptation, immunomodulation, and iron and sterol binding. Effectors resembling pathogenicity factors of plant-pathogenic oomycetes were also discovered, such as, a CBEL-like protein (possible involvement in host cell adhesion and hemagglutination), a putative RXLR effector (possibly involved in host cell modulation) and elicitin-like (ELL) proteins. Phylogenetic analysis mapped P. insidiosum ELLs to several novel clades of oomycete elicitins (ELIs), and homology modeling predicted that P. insidiosum ELLs should bind sterols. Most of the P. insidiosum ESTs showed homology to sequences in the genome or EST databases of other oomycetes, but one putative gene, with unknown function, was found to be unique to P. insidiosum. The EST dataset reported here represents the first steps in identifying genes of P. insidiosum and beginning transcriptome analysis. This genetic information will facilitate understanding of pathogenic mechanisms of this devastating pathogen. © 2011 The British Mycological Society.||en_US|
|dc.subject||Agricultural and Biological Sciences||en_US|
|dc.subject||Biochemistry, Genetics and Molecular Biology||en_US|
|dc.title||Expressed sequence tags reveal genetic diversity and putative virulence factors of the pathogenic oomycete Pythium insidiosum||en_US|
|Appears in Collections:||Scopus 2011-2015|
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