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Title: Serological response to the 2009 pandemic influenza A (H1N1) virus for disease diagnosis and estimating the infection rate in Thai population
Authors: Hatairat Lerdsamran
Chakrarat Pittayawonganon
Phisanu Pooruk
Anek Mungaomklang
Sopon Iamsirithaworn
Prasert Thongcharoen
Uraiwan Kositanont
Prasert Auewarakul
Kulkanya Chokephaibulkit
Sineenat Oota
Warin Pongkankham
Patummal Silaporn
Supaloek Komolsiri
Pirom Noisumdaeng
Tawee Chotpitayasunondh
Chariya Sangsajja
Witthawat Wiriyarat
Suda Louisirirotchanakul
Pilaipan Puthavathana
Mahidol University
Thailand Ministry of Public Health
Maharaj Nakhon Ratchasima Hospital
Thai Red Cross Agency
Division of Preventive Medicine
Bamrasnaradura Infectious Disease Institute
Keywords: Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology
Issue Date: 7-Feb-2011
Citation: PLoS ONE. Vol.6, No.1 (2011)
Abstract: Background: Individuals infected with the 2009 pandemic virus A(H1N1) developed serological response which can be measured by hemagglutination-inhibition (HI) and microneutralization (microNT) assays. Methodology/Principal Findings: MicroNT and HI assays for specific antibody to the 2009 pandemic virus were conducted in serum samples collected at the end of the first epidemic wave from various groups of Thai people: laboratory confirmed cases, blood donors and health care workers (HCW) in Bangkok and neighboring province, general population in the North and the South, as well as archival sera collected at pre- and post-vaccination from vaccinees who received influenza vaccine of the 2006 season. This study demonstrated that goose erythrocytes yielded comparable HI antibody titer as compared to turkey erythrocytes. In contrast to the standard protocol, our investigation found out the necessity to eliminate nonspecific inhibitor present in the test sera by receptor destroying enzyme (RDE) prior to performing microNT assay. The investigation in pre-pandemic serum samples showed that HI antibody was more specific to the 2009 pandemic virus than NT antibody. Based on data from pre-pandemic sera together with those from the laboratory confirmed cases, HI antibody titers ≥40 for adults and ≥20 for children could be used as the cut-off level to differentiate between the individuals with or without past infection by the 2009 pandemic virus. Conclusions/Significance: Based on the cut-off criteria, the infection rates of 7 and 12.8% were estimated in blood donors and HCW, respectively after the first wave of the 2009 influenza pandemic. Among general population, the infection rate of 58.6% was found in children versus 3.1% in adults. © 2011 Lerdsamran et al.
ISSN: 19326203
Appears in Collections:Scopus 2011-2015

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