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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/11371
Title: Genetic manipulation of Japonica rice using the OsBADH1 gene from Indica rice to improve salinity tolerance
Authors: Supaporn Hasthanasombut
Kanyaratt Supaibulwatana
Masahiro Mii
Ikuo Nakamura
Chiba University
Mahidol University
Keywords: Agricultural and Biological Sciences
Issue Date: 1-Jan-2011
Citation: Plant Cell, Tissue and Organ Culture. Vol.104, No.1 (2011), 79-89
Abstract: Betaine aldehyde dehydrogenase (BADH) is a major oxidative enzyme that converts betaine aldehyde to glycine betaine (GB), an osmoprotectant compound in plants. Japonica rice (salt-sensitive) was genetically engineered to enhance salt tolerance by introducing the OsBADH1 gene from Indica rice (salt-tolerant), which is a GB accumulator. We produced transgenic rice plants overexpressing the modified OsBADH1 gene under the control of the maize ubiquitin promoter. The transgenic rice showed increased OsBADH1 gene expression and OsBADH1 enzyme production, resulting in the accumulation of GB. It also exhibited enhanced salt tolerance in immature and mature transgenic rice seedlings. The adverse effect of salt stress on seed germination, the growth of immature and mature seedlings, water status, and photosynthetic pigments was alleviated in transgenic seedlings. © 2010 Springer Science+Business Media B.V.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=78649936030&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/11371
ISSN: 01676857
Appears in Collections:Scopus 2011-2015

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