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Title: Induction of apoptosis in murine leukemia by diarylheptanoids from Curcuma comosa Roxb.
Authors: Surawat Jariyawat
Thanapol Thammapratip
Kanoknetr Suksen
Podchanart Wanitchakool
Jintapat Nateewattana
Arthit Chairoungdua
Apichart Suksamrarn
Pawinee Piyachaturawat
Mahidol University
Ramkhamhaeng University
Keywords: Biochemistry, Genetics and Molecular Biology;Environmental Science;Pharmacology, Toxicology and Pharmaceutics
Issue Date: 1-Dec-2011
Citation: Cell Biology and Toxicology. Vol.27, No.6 (2011), 413-423
Abstract: Diarylheptanoids, isolated from the rhizome of Curcuma comosa Roxb., have several biological activities including anti-oxidant and anti-inflammation. The present study investigated the effect of five diarylheptanoids isolated from C. comosa rhizome on the proliferation of murine P388 leukemic cells. Compound-092, (3S)-1-(3,4-dihydroxyphenyl)-7-phenyl-(6E)-6-hepten-3-ol, bearing a catechol moiety, was the most potent diarylheptanoid (IC 50 of 4 μM) in inhibiting P388 leukemic cell viability by causing DNA breakage and inducing apoptosis. Apoptotic cell death was characterized by the presence of chromatin condensation, formation of apoptotic bodies, DNA fragmentation, and externalization of plasma membrane phosphatidylserine. This compound increased caspase-3 activity about fivefold above the untreated control, decreased the intracellular reduced glutathione level, and impaired mitochondrial transmembrane potential. In the presence of Cu(II) ion, the compound exhibited a pro-oxidant activity causing DNA strand breakage and enhancing the anti-proliferative activity. The results provide evidence for the pro-oxidant activity of the diarylheptanoid bearing a catechol moiety in the induction of apoptosis in murine P388 leukemia. © 2011 Springer Science+Business Media B.V.
ISSN: 15736822
Appears in Collections:Scopus 2011-2015

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