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Title: Single-molecule recognition force spectroscopy of transmembrane transporters on living cells
Authors: Theeraporn Puntheeranurak
Isabel Neundlinger
Rolf K.H. Kinne
Peter Hinterdorfer
Johannes Kepler Universitat Linz
Mahidol University
Max Planck Institut fur molekulare Physiologie
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Sep-2011
Citation: Nature Protocols. Vol.6, No.9 (2011), 1443-1452
Abstract: Atomic force microscopy (AFM) has proven to be a powerful tool in biological sciences. Its particular advantage over other high-resolution methods commonly used is that biomolecules can be investigated not only under physiological conditions but also while they perform their biological functions. Single-molecule force spectroscopy with AFM tip-modification techniques can provide insight into intermolecular forces between individual ligand-receptor pairs of biological systems. Here we present protocols for force spectroscopy of living cells, including cell sample preparation, tip chemistry, step-by-step AFM imaging, force spectroscopy and data analysis. We also delineate critical steps and describe limitations that we have experienced. The entire protocol can be completed in 12 h. The model studies discussed here demonstrate the power of AFM for studying transmembrane transporters at the single-molecule level. © 2011 Nature America, Inc. All rights reserved.
ISSN: 17502799
Appears in Collections:Scopus 2011-2015

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