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dc.contributor.authorTheeraporn Puntheeranuraken_US
dc.contributor.authorIsabel Neundlingeren_US
dc.contributor.authorRolf K.H. Kinneen_US
dc.contributor.authorPeter Hinterdorferen_US
dc.contributor.otherJohannes Kepler Universitat Linzen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherMax Planck Institut fur molekulare Physiologieen_US
dc.identifier.citationNature Protocols. Vol.6, No.9 (2011), 1443-1452en_US
dc.description.abstractAtomic force microscopy (AFM) has proven to be a powerful tool in biological sciences. Its particular advantage over other high-resolution methods commonly used is that biomolecules can be investigated not only under physiological conditions but also while they perform their biological functions. Single-molecule force spectroscopy with AFM tip-modification techniques can provide insight into intermolecular forces between individual ligand-receptor pairs of biological systems. Here we present protocols for force spectroscopy of living cells, including cell sample preparation, tip chemistry, step-by-step AFM imaging, force spectroscopy and data analysis. We also delineate critical steps and describe limitations that we have experienced. The entire protocol can be completed in 12 h. The model studies discussed here demonstrate the power of AFM for studying transmembrane transporters at the single-molecule level. © 2011 Nature America, Inc. All rights reserved.en_US
dc.rightsMahidol Universityen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleSingle-molecule recognition force spectroscopy of transmembrane transporters on living cellsen_US
Appears in Collections:Scopus 2011-2015

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