Simple jQuery Dropdowns
Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/11486
Title: Identification of Brugia malayi immunogens by an immunoproteomics approach
Authors: Sirichit Wongkamchai
Wararat Chiangjong
Supachok Sinchaikul
Shui Tein Chen
Wej Choochote
Visith Thongboonkerd
Mahidol University
Faculty of Medicine, Siriraj Hospital, Mahidol University
Academia Sinica Taiwan
Chiang Mai University
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 24-Aug-2011
Citation: Journal of Proteomics. Vol.74, No.9 (2011), 1607-1613
Abstract: Filariasis remains a health problem in tropical countries. Identification of immunogens from its causative organism would lead to development of a better diagnostic test, as well as vaccine discovery to effectively prevent this disease. We applied immunoproteomics to define potential immunogens of adult Brugia malayi that were recognized by IgM, IgG1 and IgG4 in sera of patients with four distinct clinical spectra of filariasis, including endemic asymptomatic, lymphangitis, elephantiasis and microfilaremia (n= 5/group). Sera of healthy individuals (n= 5) from non-endemic area served as the negative control. Brugian proteins were resolved by 2-DE and subjected to 2-D Western blot analysis probed with these sera. A total of 30 immunoreactive proteins recognized by IgM, IgG1 and IgG4 in sera from all four filarial groups were identified by Q-TOF MS and MS/MS analyses. Interestingly, only three immunogens were recognized by IgM in lymphangitis, elephantiasis and microfilaremia, but not in endemic asymptomatic group. IgG1 recognized 20 immunogens in endemic asymptomatic, lymphangitis and microfilaremia (mostly in endemic asymptomatic group), but not in elephantiasis, whereas IgG4 recognized 28 immunogens in all four filarial groups (mostly in microfilaremia). This large data set is an important resource for further development of a new diagnostic test and/or vaccine for filariasis. © 2011 Elsevier B.V.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=80051630272&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/11486
ISSN: 18767737
18743919
Appears in Collections:Scopus 2011-2015

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.