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Title: Determination of tafenoquine in dried blood spots and plasma using LC and fluorescence detection
Authors: Susanne Römsing
Niklas Lindegardh
Yngve Bergqvist
Hogskolan Dalarna
Uppsala Universitet
Center for Clinical Research Dalarna
Mahidol University
Nuffield Department of Clinical Medicine
Keywords: Biochemistry, Genetics and Molecular Biology;Chemistry;Health Professions;Pharmacology, Toxicology and Pharmaceutics
Issue Date: 1-Aug-2011
Citation: Bioanalysis. Vol.3, No.16 (2011), 1847-1853
Abstract: Background: The growing problem of parasites developing resistance to the traditional antimalarial drugs makes the development of new effective and safe drugs crucial. Tafenoquine is a new promising antimalarial drug for prophylaxis and treatment. Results: A bioanalytical method for the determination of tafenoquine in 100 l of capillary blood applied onto sampling paper and in 100 l of plasma has been developed and validated. The Whatman 31 ET Chr paper was treated with 0.6 mol/l tartaric acid to improve the extraction recovery and solid-phase extraction was used for cleanup procedure of the blood samples. Plasma samples were precipitated with methanol. Tafenoquine and internal standard were separated on a Zorbax SB-CN column by reversed-phase LC and detected with fluorescence detection at 262 and 470 nm. The within- and between-day variations were below 10 and 14%, respectively, over the range 50-200 nmol/l for capillary blood on sampling paper and below 6 and 10% for plasma samples. The LLOQ of the method was 50 nmol/l. Conclusion: The developed method has adequate sensitivity and is highly suitable for clinical studies in dried blood spots and plasma. © 2011 Future Science Ltd.
ISSN: 17576199
Appears in Collections:Scopus 2011-2015

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