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Title: Role of CD137 signaling in dengue virus-mediated apoptosis
Authors: Amar Nagila
Janjuree Netsawang
Chatchawan Srisawat
Sansanee Noisakran
Atthapan Morchang
Umpa Yasamut
Chunya Puttikhunt
Watchara Kasinrerk
Prida Malasit
Pa thai Yenchitsomanus
Thawornchai Limjindaporn
Mahidol University
Rangsit University
Thailand National Center for Genetic Engineering and Biotechnology
Chiang Mai University
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 8-Jul-2011
Citation: Biochemical and Biophysical Research Communications. Vol.410, No.3 (2011), 428-433
Abstract: Hepatic dysfunction is a well recognized feature of dengue virus (DENV) infection. However, molecular mechanisms of hepatic injury are still poorly understood. A complex interaction between DENV and the host immune response contributes to DENV-mediated tissue injury. DENV capsid protein (DENV C) physically interacts with the human death domain-associated protein Daxx. A double substitution mutation in DENV C (R85A/K86A) abrogates Daxx interaction, nuclear localization and apoptosis. Therefore we compared the expression of cell death genes between HepG2 cells expressing DENV C and DENV C (R85A/K86A) using a real-time PCR array. Expression of CD137, which is a membe r of the tumor necrosis factor receptor family, increased significantly in HepG2 cells expressing DENV C compared to HepG2 cells expressing DENV C (R85A/K86A). In addition, CD137-mediated apoptotic activity in HepG2 cells expressing DENV C was significantly increased by anti-CD137 antibody compared to that of HepG2 cells expressing DENV C (R85A/K86A). In DENV-infected HepG2 cells, CD137 mRNA and CD137 positive cells significantly increased and CD137-mediated apoptotic activity was increased by anti-CD137 antibody. This work is the first to demonstrate the contribution of CD137 signaling to DENV-mediated apoptosis. © 2011 Elsevier Inc.
ISSN: 10902104
Appears in Collections:Scopus 2011-2015

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