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dc.contributor.authorNarattaphol Charoenphandhuen_US
dc.contributor.authorSuparerk Laohapitakwornen_US
dc.contributor.authorKamonshanok Kraidithen_US
dc.contributor.authorLa iad Nakkrasaeen_US
dc.contributor.authorPrapaporn Jongwattanapisanen_US
dc.contributor.authorPhuntila Tharabenjasinen_US
dc.contributor.authorNateetip Krishnamraen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherKhon Kaen Universityen_US
dc.date.accessioned2018-05-03T08:02:08Z-
dc.date.available2018-05-03T08:02:08Z-
dc.date.issued2011-06-17en_US
dc.identifier.citationBiochemical and Biophysical Research Communications. Vol.409, No.4 (2011), 775-779en_US
dc.identifier.issn10902104en_US
dc.identifier.issn0006291Xen_US
dc.identifier.other2-s2.0-79959242266en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79959242266&origin=inwarden_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/11531-
dc.description.abstractParathyroid hormone (PTH) was recently demonstrated to enhance the HCO3- secretion through the apical anion channel, cystic fibrosis transmembrane conductance regulator (CFTR), but how the HCO3- entered the epithelial cells was not well understood, in part, due to the lack of specific inhibitors of the basolateral HCO3- transporters. Moreover, the function of the PTH-stimulated HCO3- secretion has never been investigated in vivo. Here, we designed three specific pairs of small interfering RNA sequences to simultaneously knockdown three variants of the electrogenic Na + /HCO3- co-transporter (NBCe)-1 in the intestinal epithelium-like Caco-2 monolayer. The results showed that NBCe1 mRNA levels were markedly reduced, and the PTH-induced transepithelial current and voltage changes were diminished after triple knockdown as determined by quantitative real-time PCR and Ussing chamber technique, respectively. An in vivo ligated intestinal loop study further showed that there was an increased fluid secretion, presumably driven by HCO3- transport, in the ileum, but not in jejunum or colon, of rats administered intravenously with 2μg/kg body weight of rat PTH 1-34. Therefore, the present results suggested that PTH stimulated intestinal HCO3- secretion, particularly in the ileum, by inducing the basolateral HCO3- uptake via NBCe1. © 2011 Elsevier Inc.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79959242266&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleElectrogenic Na<sup>+</sup>/HCO<inf>3</inf><sup>-</sup>co-transporter-1 is essential for the parathyroid hormone-stimulated intestinal HCO<inf>3</inf><sup>-</sup>secretionen_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1016/j.bbrc.2011.05.087en_US
Appears in Collections:Scopus 2011-2015

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