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dc.contributor.authorWararat Chiangjongen_US
dc.contributor.authorSupachok Sinchaikulen_US
dc.contributor.authorShui Tein Chenen_US
dc.contributor.authorVisith Thongboonkerden_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherGenomics Research Center, Academia Sinicaen_US
dc.contributor.otherNational Taiwan Universityen_US
dc.date.accessioned2018-05-03T08:02:21Z-
dc.date.available2018-05-03T08:02:21Z-
dc.date.issued2011-06-01en_US
dc.identifier.citationMolecular BioSystems. Vol.7, No.6 (2011), 1917-1925en_US
dc.identifier.issn17422051en_US
dc.identifier.issn1742206Xen_US
dc.identifier.other2-s2.0-79956081200en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79956081200&origin=inwarden_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/11543-
dc.description.abstractCalcium oxalate dihydrate (COD) crystals can adhere onto the apical surface of renal tubular epithelial cells. This process is associated with crystal growth and aggregation, resulting in kidney stone formation. Glycoproteins have been thought to play roles in response to crystal adhesion. However, components of the glycoproteome that are involved in this cellular response remain largely unknown. Our present study therefore aimed to identify altered glycoproteins upon COD crystal adhesion onto tubular epithelial cells representing distal nephron, the initiating site of kidney stone formation. Madin-Darby Canine Kidney (MDCK) cells were maintained in culture medium with or without COD crystals for 48 h (n = 5 flasks per group). Cellular proteins were extracted, resolved by 2-DE and visualized by SYPRO Ruby total protein stain, whereas glycoproteins were detected by Pro-Q Emerald glycoprotein dye. Spot matching and quantitative intensity analysis revealed 16 differentially expressed glycoprotein spots, whose corresponding total protein levels were not changed by COD crystal adhesion. These altered glycoproteins were successfully identified by Q-TOF MS and/or MS/MS analyses, and potential glycosylation sites were identified by the GlycoMod tool. For example, glycoforms of three proteasome subunits (which have a major role in regulating cell-cell dissociation) were up-regulated, whereas a glycoform of actin-related protein 3 (ARP3) (which plays an important role in cellular integrity) was down-regulated. These coordinated changes implicate that COD crystal adhesion induced cell dissociation and declined cellular integrity in the distal nephron. Our findings provide some novel insights into the pathogenic mechanisms of kidney stone disease at the molecular level, particularly cell-crystal interactions. © 2011 The Royal Society of Chemistry.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79956081200&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleCalcium oxalate dihydrate crystal induced changes in glycoproteome of distal renal tubular epithelial cellsen_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1039/c1mb05052den_US
Appears in Collections:Scopus 2011-2015

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