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Title: A genome scan for quantitative trait loci affecting cyanogenic potential of cassava root in an outbred population
Authors: Sukhuman Whankaew
Supannee Poopear
Supanath Kanjanawattanawong
Sithichoke Tangphatsornruang
Opas Boonseng
David A. Lightfoot
Kanokporn Triwitayakorn
Mahidol University
Thailand National Center for Genetic Engineering and Biotechnology
Thailand Ministry of Agriculture and Cooperatives
Southern Illinois University at Carbondale
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 25-May-2011
Citation: BMC Genomics. Vol.12, (2011)
Abstract: Background: Cassava (Manihot esculenta Crantz) can produce cyanide, a toxic compound, without self-injury. That ability was called the cyanogenic potential (CN). This project aimed to identify quantitative trait loci (QTL) associated with the CN in an outbred population derived from 'Hanatee' × ' Huay Bong 60', two contrasting cultivars. CN was evaluated in 2008 and in 2009 at Rayong province, and in 2009 at Lop Buri province, Thailand. CN was measured using a picrate paper kit. QTL analysis affecting CN was performed with 303 SSR markers.Results: The phenotypic values showed continuous variation with transgressive segregation events with more (115 ppm) and less CN (15 ppm) than either parent ('Hanatee' had 33 ppm and 'Huay Bong 60' had 95 ppm). The linkage map consisted of 303 SSR markers, on 27 linkage groups with a map that encompassed 1,328 cM. The average marker interval was 5.8 cM. Five QTL underlying CN were detected. CN08R1from 2008 at Rayong, CN09R1and CN09R2 from 2009 at Rayong, and CN09L1 and CN09L2 from 2009 at Lop Buri were mapped on linkage group 2, 5, 10 and 11, respectively. Among all the identified QTL, CN09R1 was the most significantly associated with the CN trait with LOD score 5.75 and explained the greatest percentage of phenotypic variation (%Expl.) of 26%.Conclusions: Five new QTL affecting CN were successfully identified from 4 linkage groups. Discovery of these QTL can provide useful markers to assist in cassava breeding and studying genes affecting the trait. © 2011 Whankaew et al; licensee BioMed Central Ltd.
ISSN: 14712164
Appears in Collections:Scopus 2011-2015

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