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Title: In vivo identification of Bacillus thuringiensis Cry4Ba toxin receptors by RNA interference knockdown of glycosylphosphatidylinositol-linked aminopeptidase N transcripts in Aedes aegypti larvae
Authors: Suchada Saengwiman
Aratee Aroonkesorn
Plaipol Dedvisitsakul
Somsri Sakdee
Somphob Leetachewa
Chanan Angsuthanasombat
Kusol Pootanakit
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 22-Apr-2011
Citation: Biochemical and Biophysical Research Communications. Vol.407, No.4 (2011), 708-713
Abstract: Bacillus thuringiensis Cry4Ba toxin selectively kills Aedes aegypti mosquito larvae as it is in part due to the presence of specific membrane-bound protein receptors. In this study, using data mining approach, we initially identified three potential glycosylphosphatidylinositol-linked aminopeptidase N (GPI-APN) isoforms, APN2778, APN2783 and APN5808, which are believed to act as Cry4Ba toxin receptors. These three isoforms that are functionally expressed in the larval midgut can be sequence-specific knocked down (ranging from ~80% to 95%) by soaking the Aedes aegypti larvae in buffer of long double-stranded GPI-APN RNAs (~300-680. bp). Finally, to see the physiological effect of APN knockdowns, the larvae were fed with Escherichia coli expressing Cry4Ba toxin. The results revealed that all the three identified GPI-APN isoforms may possibly function as a Cry4Ba receptor, particularly for APN2783 as those larvae with this transcript knockdown showed a dramatic increase in resistance to Cry4Ba toxicity. © 2011 Elsevier Inc.
ISSN: 10902104
Appears in Collections:Scopus 2011-2015

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