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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/11605
Title: Development of surface plasmon resonance imaging for detection of Acidovorax avenae subsp. citrulli (Aac) using specific monoclonal antibody
Authors: Chokchai Puttharugsa
Thidarat Wangkam
Nongluck Huangkamhang
Oraprapai Gajanandana
Orawan Himananto
Boonsong Sutapun
Ratthasart Amarit
Armote Somboonkaew
Toemsak Srikhirin
Mahidol University
Thailand National Center for Genetic Engineering and Biotechnology
Thailand National Electronics and Computer Technology Center
Keywords: Biochemistry, Genetics and Molecular Biology;Chemistry;Engineering
Issue Date: 15-Jan-2011
Citation: Biosensors and Bioelectronics. Vol.26, No.5 (2011), 2341-2346
Abstract: An immunosensor based on surface plasmon resonance imaging (SPR imaging) using a specific monoclonal antibody 11E5 (MAb 11E5) was developed for the detection of the seed-borne bacterium Acidovorax avenae subsp. citrulli (Aac), which causes fruit blotch in watermelons and cantaloupes, and compared to the conventional ELISA technique. The 1:40 mixed self-assembled monolayer (mixed SAM) surface was used for the immobilized MAb 11E5 on sensor surface for the detection of Aac. Both whole cells and broken cells of Aac were tested by using direct and sandwich detection assay. The limit of detection (LOD) of Aac using the SPR imaging technique and a direct detection assay was 10 6 cfu/ml and a subsequent amplification of the SPR signal using a polyclonal antibody (PAb) lowered the LOD to 5×10 5 cfu/ml. The LOD for the ELISA technique was 5×10 4 cfu/ml for the detection of Aac, which was slightly better than that for the SPR technique. However, the sensor surface based on SPR imaging offered a major advantage in terms of surface regeneration, allowing at least five cycles with a shorter time assay, multi-channel analysis with an application on multiplex detection, and an ease of the surface usage for the detection of Aac in the naturally infected plant. The surface was tested against the naturally infected sample and showed good selectivity toward the Aac bacteria. © 2010 Elsevier B.V.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=78650609435&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/11605
ISSN: 09565663
Appears in Collections:Scopus 2011-2015

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