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Title: | Cells producing insulin-like androgenic gland hormone of the giant freshwater prawn, Macrobrachium rosenbergii, proliferate following bilateral eyestalk-ablation |
Authors: | Ittipon Phoungpetchara Yotsawan Tinikul Jaruwan Poljaroen Charoonroj Chotwiwatthanakun Rapeepun Vanichviriyakit Morakot Sroyraya Peter J. Hanna Prasert Sobhon Mahidol University Deakin University |
Keywords: | Biochemistry, Genetics and Molecular Biology |
Issue Date: | 1-Jan-2011 |
Citation: | Tissue and Cell. Vol.43, No.3 (2011), 165-177 |
Abstract: | We found that the androgenic gland (AG) of Macrobrachium rosenbergii possesses three cell types. Type I cells are small polygonal shaped-cells (13.4. μm in diameter), stain strongly with hematoxylin-eosin (H & E), have abundant multilayered rough endoplasmic reticulum (rER), and nuclei containing mostly heterochromatin. Type II cells are slightly larger (18.6. μm in diameter), stain lightly with H & E, have rER with dilated cisternae, and nuclei containing mostly euchromatin. Type III cells (previously undescribed) are similar in size and shape to type I cells, but the cytoplasm is unstained and they have a high amount of smooth endoplasmic reticulum (sER) and mitochondria with tubular cristae. Bilateral eyestalk-ablation resulted in AG hypertrophy with a proliferation and predominance of type I cells as determined by bromodeoxyuridine (BrdU) assays. Expression of insulin-like androgenic gland hormone (Mr-IAG), determined by immunohistochemistry, was weak in type I cells, strong in type II cells of both the intact and eyestalk-ablated, and negative in type III cells. It was also detected in spermatogonia, nurse cells, and epithelium lining of the spermatic duct. The function of Mr-IAG in these tissues is yet to be elucidated but the distribution implies a strong role in male reproduction. © 2011 Elsevier Ltd. |
URI: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79960221505&origin=inward http://repository.li.mahidol.ac.th/dspace/handle/123456789/11615 |
ISSN: | 15323072 00408166 |
Appears in Collections: | Scopus 2011-2015 |
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