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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/11872
Title: Investigation of HC04 human hepatocyte cells interaction with fibronectin and gelatin plated on paper for cell based sensor application
Authors: U. Waiwijit
A. Wisitsoraat
A. Tuantranont
B. Oonkhanond
W. Kandhavivorn
Thailand National Electronics and Computer Technology Center
Mahidol University
Keywords: Engineering
Issue Date: 1-Dec-2011
Citation: BMEiCON-2011 - 4th Biomedical Engineering International Conference. (2011), 93-95
Abstract: Recently, paper based biosensors have been studied in the fields of biology, chemistry, pharmacology and medicine. The main advantages of paper based biosensors are low cost, biocompatibility, portability and environmental compatibility. The process of cell immobilization on paper is an important step for the development of disposable paper based biosensors with a whole-cell electrochemical electrode. The major problem of this process is poor cell viability on paper. In this work, we study the viability of HC04 human hepatocyte cells immobilized on filter papers coated with gelatin and fibronectin. HC04 cells were cultured on uncoated and coated papers in a 5% CO 2 incubator for 6 and 24 hrs. Cell viability was then detected by tryphan blue and propidium iodide staining. The results show that % cell viability on gelatin and fibronectin coated paper are more than that on uncoated one. Therefore, the developed gelatin and fibronectin coated paper is promising for cell based biosensor applications. © 2011 IEEE.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84860483312&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/11872
Appears in Collections:Scopus 2011-2015

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