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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/11882
Title: Detection of Mycobacterium tuberculosis by using loop-mediated isothermal amplification combined with a lateral flow dipstick biosensor
Authors: Thongchai Kaewphinit
Somchai Santiwatanakul
Pronpan Jaratsing
Kosum Chansiri
Narong Arunrut
Wansika Kiatpathomchai
Srinakharinwirot University
Thailand National Center for Genetic Engineering and Biotechnology
Mahidol University
Keywords: Engineering
Issue Date: 1-Dec-2011
Citation: BMEiCON-2011 - 4th Biomedical Engineering International Conference. (2011), 86-88
Abstract: Tuberculosis is a persistent problem in the developing world and the biggest cause of mortality. Loop-mediated isothermal amplification (LAMP) allows DNA to be amplified rapidly at a constant temperature. Here a LAMP method was combined with a chromatographic lateral-flow dipstick (LFD) to detect IS6110 gene of M. tuberculosis rapidly and specifically. The reaction was optimized at 65°C for 90 min and amplified DNA hybridized to an FITC-labeled oligonucleotide probe for 5 min was detected at LFD test line 5 min after application. Excluding for the step of DNA extraction, test results could be generated within 1 h 40 min. In addition to advantages of short assay time, confirmation of amplicon identity by hybridization and elimination of electrophoresis with carcinogenic ethidium bromide, the LAMP-LFD was more sensitive than an existing PCR assay for detection of M. tuberculosis. © 2011 IEEE.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84860449445&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/11882
Appears in Collections:Scopus 2011-2015

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