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|Title:||Successful derivation and characteristics of xeno-free mesenchymal stem cell lines from human amniotic fluid generated under allogenic cord blood serum supplementation|
|Citation:||Tissue Engineering and Regenerative Medicine. Vol.8, No.2 (2011), 216-223|
|Abstract:||Mesenchymal stem cells (MSC) are of interest for clinical therapy applications in many disorders. Providing MSC lines that are completely free of xeno contamination is needed in clinical level in order to reduce the occurrence of non-human pathogenic transmission and graft-rejection. Here we generate complete xeno-free MSC lines using cells from human amniotic fluid (hAF-MSC) and characterized them compared to lines generated using standard culture condition. Ten samples of amniotic fluid were used for MSC isolation to create clonal hAF-MSC lines. The cell lines were derived and cryopreserved under culture mediums supplemented with either human cord blood serum or ES grade-fetal bovine serum. Twenty clonal lines from both mediums were investigated for MSC specific characteristics. Our results demonstrate that every clonal hAF-MSC lines established under xeno-free conditions can grow under long-term culture ( > 15 passages) with a normal karyotype. The lines exhibit a high proliferation rate at close to the rate of lines derived using standard culture medium. The MSC-specific characteristics of xeno-free hAF-MSC lines are comparable to lines obtained using standard culture medium. However, we found that the xeno-free culture medium supplemented with allogenic hCS retains the positivity of CD90 marker during in vitro culture, while standard medium down-modulates the expression. We conclude that the xeno-free hAF-MSC lines produced under the conditions developed in this study have comparable cell potential to lines generated under standard conditions. This study supports the possibility of providing a new generation of MSC lines at a clinical level using a complete xeno-free system.|
|Appears in Collections:||Scopus 2011-2015|
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