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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/11957
Title: The difference in IL-1β, MIP-1α, IL-8 and IL-18 production between the infection of PMA activated U937 cells with recombinant vaccinia viruses inserted 2004 H5N1 influenza HA genes and NS genes
Authors: Molvibha Vongsakul
Jitra Kasisith
Pirom Noisumdaeng
Pilaipan Puthavathana
Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Dec-2011
Citation: Asian Pacific Journal of Allergy and Immunology. Vol.29, No.4 (2011), 349-356
Abstract: Background: The severity of avian influenza H5N1 disease is correlated with the ability of the virus to induce an over production of pro-inflammatory cytokines from innate immune cells. However, the role of each virus gene is unknown. To elaborate the function of each virus gene, the recombinant vaccinia virus inserted HA and NS gene from the 2004 H5N1 virus were used in the study. Methods: U937 cells and PMA activated U937 cells were infected with recombinant vaccinia virus inserted with HA or NS gene. The expressions of HA and NS proteins in cells were detected on immunofluorescence stained slides using a confocal microscope. The cytokine productions in the cell supernatant were quantitated by ELISA. Results: The recombinant vaccinia virus inserted with HA genes induces the production of IL-1β, MIP-1α, IL-8 and IL-18 cytokines from PMA activated U937 cells significantly more than cells infected with wild type vaccinia, whereas the recombinant vaccinia virus inserted with NS genes it was similar to that with the wild type vaccinia virus. However, there was no synergistic nor antagonistic effect of HA genes and NS genes in relation to cytokines production. Conclusion: Only the HA gene from the 2004 H5N1 virus induces IL-1β, MIP-1α, IL-8 and IL-18 cytokine productions from activated U937 cells. The same HA gene effect may or may not be the same in respiratory epithelial cells and this needs to be explored.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84855235658&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/11957
ISSN: 22288694
0125877X
Appears in Collections:Scopus 2011-2015

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