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Title: Subcellular localizations and time-course expression of dengue envelope and non-structural 1 proteins in human endothelial cells
Authors: Jesdaporn Poungsawai
Rattiyaporn Kanlaya
Sa nga Pattanakitsakul
Visith Thongboonkerd
Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Sep-2011
Citation: Microbial Pathogenesis. Vol.51, No.3 (2011), 225-229
Abstract: We performed subcellular fractionation of human endothelial cells (EA.hy926) after a challenge with dengue virus serotype-2 (DEN-2) at multiplicity of infection (MOI) of 10 for 2 h. The cells were fractionated into four fractions including cytosol, membrane/organelle, nucleus and cytoskeleton, and their purity was nicely confirmed by immunofluorescence staining of markers for individual subcellular compartments. Western blot analysis of dengue envelope (E) and non-structural 1 (NS1) proteins showed that E was present only in membrane/organelle fraction, whereas NS1 was present in membrane/organelle, nucleus, and cytoskeleton fractions. Time-course study showed that E was present immediately after the challenge, whereas NS1 was present after 12 h of the challenge. Our data may provide a new roadmap for studying mechanisms of replication of dengue virus and its interactions with host cells. © 2011 Elsevier Ltd.
ISSN: 10961208
Appears in Collections:Scopus 2011-2015

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