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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/12011
Title: The autoregulator receptor homologue AvaR3 plays a regulatory role in antibiotic production, mycelial aggregation and colony development of Streptomyces avermitilis
Authors: Kiyoko T. Miyamoto
Shigeru Kitani
Mamoru Komatsu
Haruo Ikeda
Takuya Nihira
Osaka University
Kitasato University
Mahidol University
Keywords: Immunology and Microbiology
Issue Date: 1-Aug-2011
Citation: Microbiology. Vol.157, No.8 (2011), 2266-2275
Abstract: The γ-butyrolactone autoregulator receptor has been shown to control secondary metabolism and/or morphological differentiation across many Streptomyces species. Streptomyces avermitilis produces an important anthelmintic agent (avermectin) and two further polyketide antibiotics, filipin and oligomycin. Genomic analysis of S. avermitilis revealed that this micro-organism has the clustered putative autoregulator receptor genes distant from the antibiotic biosynthetic gene clusters. Here, we describe the characterization of avaR3, one of the clustered receptor genes, which encodes a protein containing an extra stretch of amino acid residues that has not been found in the family of autoregulator receptors. Disruption of avaR3 resulted in markedly decreased production of avermectins, with delayed expression of avermectin biosynthetic genes, suggesting that AvaR3 positively controls the avermectin biosynthetic genes. Moreover, the disruption caused increased production of filipin without any changes in the transcriptional profile of the filipin biosynthetic genes, suggesting that filipin production is indirectly controlled by AvaR3. The avaR3 disruptant displayed fragmented growth in liquid culture and conditional morphological defects on solid medium. These findings demonstrated that AvaR3 acts as a global regulator that controls antibiotic production and cell morphology. © 2011 SGM.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79961061778&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/12011
ISSN: 13500872
Appears in Collections:Scopus 2011-2015

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