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Title: Improved culture-based detection and quantification of Burkholderia pseudomallei from soil
Authors: Trinh Thanh Trung
Adrian Hetzer
Eylin Topfstedt
Andre Göhler
Direk Limmathurotsakul
Vanaporn Wuthiekanun
Sharon J. Peacock
Ivo Steinmetz
Ernst-Moritz-Arndt-Universitat Greifswald
Mahidol University
University of Cambridge
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Jun-2011
Citation: Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.105, No.6 (2011), 346-351
Abstract: Environmental surveillance of the Gram-negative soil bacterium Burkholderia pseudomallei, the aetiological agent of melioidosis, is important in order to define human populations and livestock at risk of acquiring the infection. This study aimed to develop a more sensitive method for the detection of B. pseudomallei from soil samples in endemic areas compared with the currently used culture method based on soil dispersion in water. We report the development of a new protocol that involves soil dispersion in a polyethylene glycol (PEG) and sodium deoxycholate (DOC) solution to increase the yield of viable B. pseudomallei from soil samples. Comparative testing of soil samples from Northeast Thailand covering a w ide range of B. pseudomallei concentrations demonstrated a significantly higher recovery (P < 0.0001) of B. pseudomallei colony-forming units by the new method compared with the conventional method. The data indicate that using the detergents PEG and DOC not only results in a higher recovery of viable B. pseudomallei but also results in a shift in the bacterial species recovered from soil samples. Future studies on the geographical distribution and population structure of B. pseudomallei in soil are likely to benefit from the new protocol described here. © 2011 Royal Society of Tropical Medicine and Hygiene.
ISSN: 18783503
Appears in Collections:Scopus 2011-2015

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