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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/12057
Title: A Thermostable phytase from Neosartorya spinosa BCC 41923 and its expression in Pichia pastoris
Authors: Patcharaporn Pandee
Pijug Summpunn
Suthep Wiyakrutta
Duangnate Isarangkul
Vithaya Meevootisom
Mahidol University
Center of Excellence for Agricultural Biotechnology (AG-BIO/PERDO-CHE)
Keywords: Immunology and Microbiology
Issue Date: 1-Apr-2011
Citation: Journal of Microbiology. Vol.49, No.2 (2011), 257-264
Abstract: A phytase gene was cloned from Neosartorya spinosa BCC 41923. The gene was 1,455 bp in size, and the mature protein contained a polypeptide of 439 amino acids. The deduced amino acid sequence contains the consensus motif (RHGXRXP) which is conserved among phytases and acid phosphatases. Five possible disulfide bonds and seven potential N-glycosylation sites have been predicted. The gene was expressed in Pichia pastoris KM71 as an extracellular enzyme. The purified enzyme had specific activity of 30. 95 U/mg at 37°C and 38. 62 U/mg at 42°C. Molecular weight of the deglycosylated recombinant phytase, determined by SDS-PAGE, was approximately 52 kDa. The optimum pH and temperature for activity were pH 5. 5 and 50°C. The residual phytase activity remained over 80% of initial activity after the enzyme was stored in pH 3. 0 to 7. 0 for 1 h, and at 60% of initial activity after heating at 90°C for 20 min. The enzyme exhibited broad substrate specificity, with phytic acid as the most preferred substrate. Its K < inf > m < /inf > and V < inf > max < /inf > for sodium phytate were 1. 39 mM and 434. 78 U/mg, respectively. The enzyme was highly resistant to most metal ions tested, including Fe < sup > 2+ < /sup > , Fe < sup > 3+ < /sup > , and Al < sup > 3+ < /sup > . When incubated with pepsin at a pepsin/phytase ratio of 0. 02 (U/U) at 37°C for 2 h, 92% of its initial activity was retained. However, the enzyme was very sensitive to trypsin, as 5% of its initial activity was recovered after treating with trypsin at a trypsin/phytase ratio of 0. 01 (U/U). © 2011 The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79955720112&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/12057
ISSN: 12258873
12258873
Appears in Collections:Scopus 2011-2015

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