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dc.contributor.authorPiengchan Sonthayanonen_US
dc.contributor.authorWirongrong Chierakulen_US
dc.contributor.authorVanaporn Wuthiekanunen_US
dc.contributor.authorJanjira Thaipadungpaniten_US
dc.contributor.authorThareerat Kalambahetien_US
dc.contributor.authorSiriphan Boonsilpen_US
dc.contributor.authorPremjit Amornchaien_US
dc.contributor.authorLee D. Smytheen_US
dc.contributor.authorDirek Limmathurotsakulen_US
dc.contributor.authorNicholas P. Dayen_US
dc.contributor.authorSharon J. Peacocken_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherOrganisation Mondiale de la Santeen_US
dc.contributor.otherNuffield Department of Clinical Medicineen_US
dc.contributor.otherUniversity of Cambridgeen_US
dc.date.accessioned2018-05-03T08:17:03Z-
dc.date.available2018-05-03T08:17:03Z-
dc.date.issued2011-04-01en_US
dc.identifier.citationAmerican Journal of Tropical Medicine and Hygiene. Vol.84, No.4 (2011), 614-620en_US
dc.identifier.issn00029637en_US
dc.identifier.other2-s2.0-79958007507en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79958007507&origin=inwarden_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/12062-
dc.description.abstractThere is a lack of diagnostic tests for leptospirosis in technology-restricted settings. We developed loop-mediated isothermal amplification (LAMP) specific for the 16S ribosomal RNA gene (rrs) of pathogenic and intermediate group Leptospira species. The lower limit of detection was 10 genomic equivalents/reaction, and analytical specificity was high; we observed positive reactions for pathogenic/intermediate groups and negative reactions for non-pathogenic Leptospira species and other bacterial species. We evaluated this assay in Thailand by using a case-control study of 133 patients with laboratory-proven leptospirosis and 133 patients with other febrile illnesses. Using admission blood, we found that the rrs LAMP showed positive results in 58 of 133 cases (diagnostic sensitivity = 43.6, 95% confidence interval [CI] = 35.0-52.5) and in 22 of 133 controls (diagnostic specificity = 83.5, 95% CI = 76.0-89.3). Sensitivity was high for 39 patients who were culture positive for Leptospira spp. (84.6, 95% CI = 69.5-94.1). The rrs LAMP can provide an admission diagnosis in approximately half of patients with leptospirosis, but its clinical utility is reduced by a lower specificity. Copyright © 2011 by The American Society of Tropical Medicine and Hygiene.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79958007507&origin=inwarden_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectMedicineen_US
dc.titleAccuracy of loop-mediated isothermal amplification for diagnosis of human leptospirosis in Thailanden_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.4269/ajtmh.2011.10-0473en_US
Appears in Collections:Scopus 2011-2015

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