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Title: A prospective evaluation of diagnostic methodologies for the acute diagnosis of dengue virus infection on the Thailand-Myanmar border
Authors: Wanitda Watthanaworawit
Paul Turner
Claudia L. Turner
Ampai Tanganuchitcharnchai
Richard G. Jarman
Stuart D. Blacksell
François H. Nosten
Shoklo Malaria Research Unit
Mahidol University
University of Oxford
Armed Forces Research Institute of Medical Sciences, Thailand
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Jan-2011
Citation: Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.105, No.1 (2011), 32-37
Abstract: Clinically useful diagnostic tests of dengue virus infection are lacking. We prospectively evaluated the performance of real-time reverse transcriptase (rRT)-PCR, NS-1 antigen and IgM antibody tests to confirm dengue virus infection in acute blood specimens from 162 patients presenting with undifferentiated febrile illness compatible with dengue infection. rRT-PCR was the most sensitive test (89%) and potentially could be used as a single test for confirmation of dengue infection. NS-1 antigen and IgM antibody were not sufficiently sensitive to be used as a single confirmatory test with sensitivities of 54% and 17% respectively. The specificities of rRT-PCR, NS-1 antigen and IgM antibody tests were 96%, 100% and 88% respectively. Combining NS-1 and rRT-PCR or the combination of all three tests resulted in the highest sensitivity (93%) but specificities dropped to 96% and 83% respectively. We conclude that at least the combination of two tests, either agent detection (rRT-PCR) or antigen detection (NS-1) plus IgM antibody detection should be used for laboratory confirmation of dengue infection. © 2010 Royal Society of Tropical Medicine and Hygiene.
ISSN: 00359203
Appears in Collections:Scopus 2011-2015

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