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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/12153
Title: Molecular detection and speciation of pathogenic Leptospira spp. in blood from patients with culture-negative leptospirosis
Authors: Siriphan Boonsilp
Janjira Thaipadungpanit
Premjit Amornchai
Vanaporn Wuthiekanun
Wirongrong Chierakul
Direk Limmathurotsakul
Nicholas P. Day
Sharon J. Peacock
Mahidol University
Faculty of Medicine, Siriraj Hospital, Mahidol University
Nuffield Department of Clinical Medicine
University of Cambridge
Keywords: Medicine
Issue Date: 13-Dec-2011
Citation: BMC Infectious Diseases. Vol.11, (2011)
Abstract: Background: Pathogenic Leptospira spp. present in the blood of patients with leptospirosis during the first week of symptoms can be detected using culture or PCR. A proportion of patients who are positive by PCR are negative by culture. Leptospira spp. are fastidious bacteria, and we hypothesized that a false-negative culture result may represent infection with a distinct bacterial subset that fail to grow in standard culture medium.Methods: We evaluated our hypothesis during a prospective study of 418 consecutive patients presenting to a hospital in northeast Thailand with an acute febrile illness. Admission blood samples were taken for Leptospira culture and PCR. A single tube nested PCR that amplified a region of the rrs gene was developed and applied, amplicons sequenced and a phylogenetic tree reconstructed.Results: 39/418 (9%) patients were culture-positive for Leptospira spp., and 81/418 (19%) patients were culture-negative but rrs PCR-positive. The species associated with culture-positive leptospirosis (37 L. interrogans and 2 L. borgpetersenii) were comparable to those associated with culture-negative, PCR-positive leptospirosis (76 L. interrogans, 4 L. borgpetersenii, 1 unidentified, possibly new species).Conclusion: Molecular speciation failed to identify a unique bacterial subset in patients with culture-negative, PCR-positive leptospirosis. The rate of false-negative culture was high, and we speculate that antibiotic pre-treatment is the most likely explanation for this. © 2011 Boonsilp et al; licensee BioMed Central Ltd.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=83255164933&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/12153
ISSN: 14712334
Appears in Collections:Scopus 2011-2015

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