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Title: Determination of discriminatory power of genetic markers used for genotyping giardia duodenalis
Authors: Suradej Siripattanapipong
Saovanee Leelayoova
Mathirut Mungthin
Rc Andrew Thompson
Parima Boontanom
Wilai Saksirisamphant
Peerapan Tan-Ariya
Mahidol University
Phramongkutklao College of Medicine
Murdoch University
Chulalongkorn University
Keywords: Medicine
Issue Date: 1-Jul-2011
Citation: Southeast Asian Journal of Tropical Medicine and Public Health. Vol.42, No.4 (2011), 764-771
Abstract: Small subunit ribosomal DNA (SSU-rDNA), glutamate dehydrogenase (gdh), β-giardin, triosephosphate isomerase (tpi), and elongation factor 1-alpha (ef1-α) genes are useful genetic markers for genotypic analysis of the intestinal protozoan, Giardia duodenalis (syn. G. lamblia, G. intestinalis), the cause of enteric disease in humans. To quantitatively compare the discriminatory power of these loci, 43 fecal samples were collected from central, northern and eastern Thailand and G. duodenalis specimens were analyzed using PCR-based genotyping and subcloning methods. Approximately equal prevalence of assemblage A (21) and B (22) were present among these populations. Analysis of Simpson's index and Wallace coefficient values from assemblage B isolates together with the data obtained from GenBank showed that the combination of two loci provides a higher discrimination power for subgenotyping G. duodenalis than using any single locus.
ISSN: 01251562
Appears in Collections:Scopus 2011-2015

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