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Title: Clostridium difficile transcriptome analysis using pig ligated loop model reveals modulation of pathways not modulated in vitro
Authors: Joy Scaria
Tavan Janvilisri
Susan Fubini
Robin D. Gleed
Sean P. McDonough
Yung Fu Chang
Cornell University
Mahidol University
Keywords: Medicine
Issue Date: 1-Jun-2011
Citation: Journal of Infectious Diseases. Vol.203, No.11 (2011), 1613-1620
Abstract: A pig ligated loop model was used to analyze the in vivo transcriptome response of Clostridium difficile. Bacterial RNA from the loops was retrieved at different times and was used for microarray analysis. Several virulence-associated genes and genes involved in sporulation cascade were differentially expressed (DE). In concordance with observed upregulation of toxin genes in microarray, enzyme-linked immunosorbent assay estimation of total toxin showed high amounts of toxin in the loops. Several genes that were absent in primary annotation of C. difficile 630 but annotated in a secondary annotation were found to be DE. Pathway c omparison of DE genes in vitro and in vivo showed that when several pathways were expressed in all conditions, several of the C. difficile pathways were uniquely expressed only in vivo. The pathways observed to be modulated only in this study could be targets of new therapeutic agents against C. difficile infection. © The Author 2011. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved.
ISSN: 00221899
Appears in Collections:Scopus 2011-2015

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