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Title: Assessment and comparative analysis of a rapid diagnostic test (Tubex<sup>®</sup>) for the diagnosis of typhoid fever among hospitalized children in rural Tanzania
Authors: Benedikt Ley
Kamala Thriemer
Shaali M. Ame
George M. Mtove
Lorenz von Seidlein
Ben Amos
Ilse C E Hendriksen
Abraham Mwambuli
Aikande Shoo
Deok R. Kim
Leon R. Ochiai
Michael Favorov
John D. Clemens
Harald Wilfing
Jacqueline L. Deen
Said M. Ali
International Vaccine Institute, Seoul
Public Health Laboratory (Pemba) - Ivo de Carneri
National Institute for Medical Research Tanga
Joint Malaria Programme
Menzies School of Health Research
Teule Hospital
Mahidol University
Universitat Wien
Keywords: Medicine
Issue Date: 24-May-2011
Citation: BMC Infectious Diseases. Vol.11, (2011)
Abstract: Background: Typhoid fever remains a significant health problem in many developing countries. A rapid test with a performance comparable to that of blood culture would be highly useful. A rapid diagnostic test for typhoid fever, Tubex ® , is commercially available that uses particle separation to detect immunoglobulin M directed towards S almonella Typhi O9 lipopolysaccharide in sera.Methods: We assessed the sensitivity and specificity of the Tubex test among Tanzanian children hospitalized with febrile illness using blood culture as gold standard. Evaluation was done considering blood culture confirmed S. Typhi with non-typhi salmonella (NTS) and non - salmonella isolates as controls as well as with non-salmonella isolates only.Results: Of 139 samples tested with Tubex, 33 were positive for S. Typhi in blood culture, 49 were culture-confirmed NTS infections, and 57 were other non-salmonella infections. Thirteen hemolyzed samples were excluded. Using all non - S. Typhi isolates as controls, we showed a sensitivity of 79% and a specificity of 89%. When the analysis was repeated excluding NTS from the pool of controls we showed a sensitivity of 79% and a specificity of 97%. There was no significant difference in the test performance using the two different control groups (p > 0.05).Conclusion: This first evaluation of the Tubex test in an African setting showed a similar performance to those seen in some Asian settings. Comparison with the earlier results of a Widal test using the same samples showed no significant difference (p > 0.05) for any of the performance indicators, irrespective of the applied control group. © 2011 Ley et al; licensee BioMed Central Ltd.
ISSN: 14712334
Appears in Collections:Scopus 2011-2015

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