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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/12527
Title: Improved detection of nasopharyngeal cocolonization by multiple pneumococcal serotypes by use of latex agglutination or molecular serotyping by microarray
Authors: Paul Turner
Jason Hinds
Claudia Turner
Auscharee Jankhot
Katherine Gould
Stephen D. Bentley
François Nosten
David Goldblatt
Shoklo Malaria Research Unit
Mahidol University
University of Oxford
St George's University of London
Wellcome Trust Sanger Institute
UCL Institute of Child Health
Keywords: Medicine
Issue Date: 1-May-2011
Citation: Journal of Clinical Microbiology. Vol.49, No.5 (2011), 1784-1789
Abstract: Identification of Streptococcus pneumoniae in the nasopharynx is critical for an understanding of transmission, estimates of vaccine efficacy, and possible replacement disease. Conventional nasopharyngeal swab (NPS) culture and serotyping (the WHO protocol) is likely to underestimate multiple-serotype carriage. We compared the WHO protocol with methods aimed at improving cocolonization detection. One hundred twenty-five NPSs from an infant pneumococcal-carriage study, containing ≥1 serotype by WHO culture, were recultured in duplicate. A sweep of colonies from one plate culture was serotyped by latex agglutination. DNA extracted from the second plate was analyzed by S. pneumoniae molecular-serotyping microarray. Multiple serotypes were detected in 11.2% of the swabs by WHO culture, 43.2% by sweep serotyping, and 48.8% by microarray. Sweep and microarray were more likely to detect multiple serotypes than WHO culture (P < 0.0001). Cocolonization detection rates were similar between microarray and sweep, but the microarray identified the greatest number of serotypes. A common serogroup type was identified in 95.2% of swabs by all methods. WHO methodology significantly underestimates multiple-serotype carriage compared to these alternate methods. Sweep serotyping is cost-effective and field deployable but may fail to detect serotypes at low abundance, whereas microarray serotyping is more costly and technology dependent but may detect these additional minor carried serotypes. Copyright © 2011, American Society for Microbiology. All Rights Reserved.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79955507600&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/12527
ISSN: 1098660X
00951137
Appears in Collections:Scopus 2011-2015

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