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Title: Enhancement of recombinant human BMP-7 bone formation with bmp binding peptide in a rodent femoral defect model
Authors: Jen Chung Liao
Shiau Tzu Tzeng
Gun Keorochana
Kwang Bok Lee
Jared S. Johnson
Yuichiro Morishita
Samuel S. Murray
Jeffrey C. Wang
Chang Gung University
David Geffen School of Medicine at UCLA
Tzu Chi University
Mahidol University
Chonbuk National University, School of Medicine
VA Medical Center
Keywords: Medicine
Issue Date: 1-May-2011
Citation: Journal of Orthopaedic Research. Vol.29, No.5 (2011), 753-759
Abstract: Bone morphogenetic binding peptide (BBP) is an 18.5 kDa fragment of a bone matrix protein peptide. A rat femoral defect model was used to test the effect of BBP combined with recombinant human bone morphogenetic protein-7 (rhBMP-7) to induced bone healing. Two doses of BBP (500 and 1000 μg) were tested with two doses of rhBMP-7 (2 and 5 μg), and the results were c ompared with a positive control (10 μg rhBMP-7). Bone healing was evaluated by radiology, manual palpation, microcomputed tomography, and histology. The high dose of 10 μg of rhBMP-7 resulted in a consistent 100% bone union rate and a mature histological appearance on histology, and was used as a positive control. When 1000 μg of BBP was combined with lower doses of BMP-7 (2 μg rhBMP-7 or 5 μg rhBMP-7) significant differences were seen in radiographic scores, manual palpation, and bone volume, when compared to 2 μg rhBMP-7 or 5 μg rhBMP-7 alone. The combination of 1000 μg of BBP and 5 μg rhBMP-7 also achieved 100% fusion rate, induced a larger amount of bone formation, and yielded similar maturity of bone marrow when compared with the high dosage 10 μg rhBMP-7 group. This study demonstrated that when combined together, BBP can enhance the bone healing of rhBMP-7. Improved healing imparted by the addition of BBP may result in lesser amounts of rhBMP-7 needed to achieve union in the clinical setting. Copyright © 2010 Orthopaedic Research Society.
ISSN: 1554527X
Appears in Collections:Scopus 2011-2015

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