Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/12693
Title: Exposure of Acinetobacter baylyi ADP1 to the biocide chlorhexidine leads to acquired resistance to the biocide itself and to oxidants
Authors: Mayuree Fuangthong
Mudcharee Julotok
Worasamon Chintana
Kristin Kuhn
Surawach Rittiroongrad
Paiboon Vattanaviboon
Skorn Mongkolsuk
Chulabhorn Research Institute
Chulabhorn Graduate Institute
Mahidol University
Massachusetts Institute of Technology
Center of Excellence on Environmental Health
Armed Forces Research Institute of Medical Sciences, Thailand
Keywords: Medicine;Pharmacology, Toxicology and Pharmaceutics
Issue Date: 1-Feb-2011
Citation: Journal of Antimicrobial Chemotherapy. Vol.66, No.2 (2011), 319-322
Abstract: Objectives: The aim of this study was to determine the effect of exposure to sublethal concentrations of chlorhexidine on oxidative stress protection by Acinetobacter baylyi ADP1. Methods: ADP1 cultures were exposed to sublethal concentrations of chlorhexidine prior to being challenged with lethal concentrations of chlorhexidine itself and by oxidants. Oxidant-sensitive dyes and a flow cytometer were used to measure the formation of reactive oxygen species. The role of efflux pumps in chlorhexidine resistance was investigated using a specific inhibitor. Results: Exposure of ADP1 to low concentrations of chlorhexidine induced adaptive and cross-protective responses to chlorhexidine and oxidants (H 2 O 2 and a superoxide anion generator), respectively. Chlorhexidine treatment of ADP1 resulted in the formation of H 2 O 2 and superoxide anions that are probably responsible for the cross-protection against oxidants. Conclusions: Exposure of ADP1 to sublethal concentrations of chlorhexidine confers inducible resistance to lethal concentrations of chlorhexidine itself and to oxidants. An important link was demonstrated between exposure to a biocide and the gaining of resistance to both the biocide and oxidative stress. © The Author 2010. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=78651416502&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/12693
ISSN: 14602091
03057453
Appears in Collections:Scopus 2011-2015

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