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dc.contributor.authorSunee Channarongen_US
dc.contributor.authorWanpen Chaicumpaen_US
dc.contributor.authorNuttanan Sinchaipaniden_US
dc.contributor.authorAmpol Mitrevejen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherFaculty of Medicine, Thammasat Universityen_US
dc.identifier.citationAAPS PharmSciTech. Vol.12, No.1 (2011), 192-200en_US
dc.description.abstractThe aim of this study was to develop chitosan-coated and polyplex-loaded liposomes (PLLs) containing DNA vaccine for Peyer's patch targeting. Plain liposomes carrying plasmid pRc/CMV-HBs were prepared by the reverse-phase evaporation method. Chitosan coating was carried out by incubation of the liposomal suspensions with chitosan solution. Main lipid components of liposomes were phosphatidylcholine/cholesterol. Sodium deoxycholate and dicetyl phosphate were used as negative charge inducers. The zeta potentials of plain liposomes were strongly affected by the pH of the medium. Coating with chitosan variably increased the surface charges of the liposomes. To increase the zeta potential and stability of the liposome, chitosan was also used as a DNA condensing agent to form a polyplex. The PLLs were coated with chitosan solution. In vivo study of PLLs was carried out in comparison with chitosan-coated liposomes using plasmid encoding green fluorescence protein as a reporter. A single dose of plasmid equal to 100 μg was intragastrically inoculated into BALB/c mice. The expression of green fluorescence protein (GFP) was detected after 24 h using a confocal laser scanning microscope. The signal of GFP was obtained from positively charged chitosan-coated liposomes but found only at the upper part of duodenum. With chitosan-coated PLL540, the signal of GFP was found throughout the intestine. Chitosan-coated PLL demonstrated a higher potential to deliver the DNA to the distal intestine than the chitosan-coated liposomes due to the increase in permanent positive surface charges and the decreased enzymatic degradation. © 2010 American Association of Pharmaceutical Scientists.en_US
dc.rightsMahidol Universityen_US
dc.subjectPharmacology, Toxicology and Pharmaceuticsen_US
dc.titleDevelopment and evaluation of chitosan-coated liposomes for oral DNA vaccine: The improvement of Peyer's patch targeting using a polyplex-loaded liposomesen_US
Appears in Collections:Scopus 2011-2015

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