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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/13350
Title: Multiploid CD61+ Cells Are the Pre-Dominant Cell Lineage Infected during Acute Dengue Virus Infection in Bone Marrow
Authors: Kristina B. Clark
Sansanee Noisakran
Nattawat Onlamoon
Hui Mien Hsiao
John Roback
Francois Villinger
Aftab A. Ansari
Guey Chuen Perng
Emory University School of Medicine
Thailand National Center for Genetic Engineering and Biotechnology
Mahidol University
Emory University
National Cheng Kung University
Keywords: Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology;Medicine
Issue Date: 27-Dec-2012
Citation: PLoS ONE. Vol.7, No.12 (2012)
Abstract: Depression of the peripheral blood platelet count during acute infection is a hallmark of dengue. This thrombocytopenia has been attributed, in part, to an insufficient level of platelet production by megakaryocytes that reside in the bone marrow (BM). Interestingly, it was observed that dengue patients experience BM suppression at the onset of fever. However, few studies focus on the interaction between dengue virus (DENV) and megakaryocytes and how this interaction can lead to a reduction in platelets. In the studies reported herein, BM cells from normal healthy rhesus monkeys (RM) and humans were utilized to identify the cell lineage(s) that were capable of supporting virus infection and replication. A number of techniques were employed in efforts to address this issue. These included the use of viral RNA quantification, nonstructural protein and infectivity assays, phenotypic studies utilizing immunohistochemical staining, anti-differentiation DEAB treatment, and electron microscopy. Cumulative results from these studies revealed that cells in the BM were indeed highly permissive for DENV infection, with human BM having higher levels of viral production compared to RM. DENV-like particles were predominantly observed in multi-nucleated cells that expressed CD61+. These data suggest that megakaryocytes are likely the predominant cell type infected by DENV in BM, which provides one explanation for the thrombocytopenia and the dysfunctional platelets characteristic of dengue virus infection. © 2012 Clark et al.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84871669031&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/13350
ISSN: 19326203
Appears in Collections:Scopus 2011-2015

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