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dc.contributor.authorPimsiri Danphitsanuparnen_US
dc.contributor.authorPanitch Boonsnongcheepen_US
dc.contributor.authorThanaphol Boriboonkaseten_US
dc.contributor.authorYupyn Chintapakornen_US
dc.contributor.authorSompop Prathanturarugen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherChulalongkorn Universityen_US
dc.identifier.citationPlant Cell, Tissue and Organ Culture. Vol.111, No.3 (2012), 315-322en_US
dc.description.abstractUsing several explants of Pueraria candollei Grah. ex Benth. var. candollei and two strains of Agrobacterium rhizogenes (ATCC 15834 and 43057), hairy root cultures were established. Including 100 μM acetosyringone in the culture medium enhanced frequency of hairy root induction by up to 58 %. Subsequently, effects of inoculum size (IS) and temperature on growth and production of isoflavonoids in hairy roots were determined. Conditions of 1 % IS and 32 °C promoted the highest accumulation of total isoflavonoid content, up to 31. 0 ± 22. 6 mg/g dry weight (DW), in hairy roots. Moreover, culture of hairy roots at 32 °C decreased browning of hairy roots. Furthermore, this temperature promoted accumulation of the secondary metabolite daidzein; whereas, hairy root cultures at the stationary phase accumulated higher amounts of the isoflavonoid puerarin rather than daidzein. © 2012 Springer Science+Business Media B.V.en_US
dc.rightsMahidol Universityen_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.titleEffects of Agrobacterium rhizogenes strains and other parameters on production of isoflavonoids in hairy roots of Pueraria candollei Grah. ex Benth. var. candolleien_US
Appears in Collections:Scopus 2011-2015

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