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|Title:||Complete genome sequence of virulence-enhancing siphophage VHS1 from Vibrio harveyi|
Timothy W. Flegel
Thailand National Center for Genetic Engineering and Biotechnology
Center of Excellence for Agricultural Biotechnology (AG-BIO/PERDO-CHE)
|Keywords:||Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology;Environmental Science;Immunology and Microbiology|
|Citation:||Applied and Environmental Microbiology. Vol.78, No.8 (2012), 2790-2796|
|Abstract:||Vibrio harveyi siphophage 1 (VHS1) is a tailed phage with an icosahedral head of approximately 66 nm in diameter and an unornamented, flexible tail of approximately 153 nm in length. When Vibrio harveyi 1114GL is lysogenized with VHS1, its virulence for the black tiger shrimp (Penaeus monodon) increases by more than 100 times, and this coincides with production of a toxin(s) associated with shrimp hemocyte agglutination. Curiously, the lysogen does not show increased virulence for the whiteleg shrimp (Penaeus [Litopenaeus] vannamei). Here we present and annotate the complete, circular genome of VHS1 (81,509 kbp; GenBank accession number JF713456). By software analysis, the genome contains 125 putative open reading frames (ORFs), all of which appear to be located on the same DNA strand, similar to the case for many other bacteriophages. Most of the putative ORFs show no significant homology to known sequences in GenBank. Notable exceptions are ORFs for a putative DNA polymerase and putative phage structural proteins, including a portal protein, a phage tail tape measure protein, and a phage head protein. The last protein was identified as a component of the species-specific toxin mixture described above as being associated with agglutination of hemocytes from P. monodon. © 2012, American Society for Microbiology.|
|Appears in Collections:||Scopus 2011-2015|
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