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Title: Composition and localization of lipids in penaeus merguiensis ovaries during the ovarian maturation cycle as revealed by imaging mass spectrometry
Authors: Piyachat Chansela
Naoko Goto-Inoue
Nobuhiro Zaima
Takahiro Hayasaka
Morakot Sroyraya
Napamanee Kornthong
Attakorn Engsusophon
Montakan Tamtin
Chatchawalee Chaisri
Prasert Sobhon
Mitsutoshi Setou
Mahidol University
Hamamatsu University School of Medicine
Tokyo Metropolitan University
Kindai University
Coastal Fisheries Research and Development Bureau
Keywords: Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology
Issue Date: 14-Mar-2012
Citation: PLoS ONE. Vol.7, No.3 (2012)
Abstract: Ovary maturation, oocyte differentiation, and embryonic development in shrimp are highly dependent on nutritional lipids taken up by female broodstocks. These lipids are important as energy sources as well as for cell signaling. In this study, we report on the compositions of major lipids, i.e. phosphatidylcholines (PCs), triacylglycerols (TAGs), and fatty acids (FAs), in the ovaries of the banana shrimp, Penaeus merguiensis, during ovarian maturation. Thin-layer chromatography analysis showed that the total PC and TAG signal intensities increased during ovarian maturation. Further, by using gas chromatography, we found that (1) FAs 14:0, 16:1, 18:1, 18:2, 20:1, and 22:6 proportionally increased as ovarian development progressed to more mature stages; (2) FAs 16:0, 18:0, 20:4, and 20:5 proportionally decreased; and (3) FAs 15:0, 17:0, and 20:2 remained unchanged. By using imaging mass spectrometry, we found that PC 16:0/16:1 and TAG 18:1/18:2/22:6 were detected in oocytes stages 1 and 2. PCs 16:1/20:4, 16:0/22:6, 18:3/22:6, 18:1/22:6, 20:5/22:6, and 22:6/22:6 and TAGs 16:0/16:1/18:3, 16:0/18:1/18:3, 16:0/18:1/18:1, and 16:0/18:2/22:6 were present in all stages of oocytes. In contrast, the PC- and TAG-associated FAs 20:4, 20:5, and 22:6 showed high signal intensities in stage 3 and 4 oocytes. These FAs may act as nutrition sources as well as signaling molecules for developing embryos and the hatching process. Knowledge of lipid compositions and localization could be helpful for formulating the diet for female broodstocks to promote fecundity and larval production. © 2012 Chansela et al.
ISSN: 19326203
Appears in Collections:Scopus 2011-2015

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