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|Title:||In vitro acanthamoebicidal activity of fusaric acid and dehydrofusaric acid from an endophytic fungus fusarium spTlau3|
Araya Dharmkrong-At Chusattayanond
Chulabhorn Research Institute
Chulabhorn Graduate Institute
Eastern Asia University
Thailand National Center for Genetic Engineering and Biotechnology
|Keywords:||Biochemistry, Genetics and Molecular Biology;Chemistry;Medicine;Pharmacology, Toxicology and Pharmaceutics|
|Citation:||Planta Medica. Vol.78, No.14 (2012), 1562-1567|
|Abstract:||Acanthamoeba is a genus of free-living protozoa that can cause sight- and life-threatening diseases in man. Its control is still problematic due to the lack of effective and nontoxic acanthamoebicidal agents. Herein, we report the first finding of an in vitro killing effect of fusaric acid and dehydrofusaric acid, isolated from metabolites of the Fusarium fujikuroi species complex Tlau3, on Acanthamoeba trophozoites isolated from two clinical (AS, AR) and two soil (S3, S5) samples. AS, AR, and S3 were classified as members of the T4 genotype, whereas S5 belongs to T5. The fungal extract was found to exhibit acanthamoebicidal activity, and activity-guided fractionation led to the isolation and identification of active principles, fusaric acid and dehydrofusaric acid. Their effects were in concentration- and time-dependent manners. Fusaric acid and dehydrofusaric acid showed ICvalues against AS trophozoites of 0.31 and 0.34M, respectively. Commercial fusaric acid displayed the same acanthamoebicidal activity as that of the isolated fusaric acid, and therefore, commercial fusaric acid was used throughout this study. ICvalues of commercial fusaric acid against AR, S3, and S5 trophozoites were 0.33, 0.33, and 0.66M, respectively. Fusaric acid calcium salt has a history of usage as a hypotensive agent in humans with no observed toxicity. The present study suggests that fusaric acid may serve as a starting point for the development towards therapeutic and environmental acanthamoebicides with low toxicity to humans. © 2012 Georg Thieme Verlag KG Stuttgart New York.|
|Appears in Collections:||Scopus 2011-2015|
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