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Title: DNA Sequence Preferences of Transcriptional Activators Correlate More Strongly than Repressors with Nucleosomes
Authors: Varodom Charoensawan
Sarath Chandra Janga
Martha L. Bulyk
M. Madan Babu
Sarah A. Teichmann
The Medical Research Council Laboratory of Molecular Biology
Mahidol University
Indiana University-Purdue University Indianapolis
Indiana University School of Medicine Indianapolis
Brigham and Women's Hospital and Harvard Medical School
The Harvard-MIT Division of Health Sciences and Technology
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 27-Jul-2012
Citation: Molecular Cell. Vol.47, No.2 (2012), 183-192
Abstract: Transcription factors (TFs) and histone octamers are two abundant classes of DNA binding proteins that coordinate the transcriptional program in cells. Detailed studies of individual TFs have shown that TFs bind to nucleosome-occluded DNA sequences and induce nucleosome disruption/repositioning, while recent global studies suggest this is not the only mechanism used by all TFs. We have analyzed to what extent the intrinsic DNA binding preferences of TFs and histones play a role in determining nucleosome occupancy, in addition to nonintrinsic factors such as the enzymatic activity of chromatin remodelers. The majority of TFs in budding yeast have an intrinsic sequence preference overlapping with nucleosomal histones. TFs with intrinsic DNA binding properties highly correlated with those of histones tend to be associated with gene activation and might compete with histones to bind to genomic DNA. Consistent with this, we show that activators induce more nucleosome disruption upon transcriptional activation than repressors. © 2012 Elsevier Inc.
ISSN: 10974164
Appears in Collections:Scopus 2011-2015

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