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Title: Expression of the male reproduction-related gene (Mar-Mrr) in the spermatic duct of the giant freshwater prawn, Macrobrachium rosenbergii
Authors: Ittipon Phoungpetchara
Yotsawan Tinikul
Jaruwan Poljaroen
Narin Changklungmoa
Tanapan Siangcham
Morakot Sroyraya
Charoonroj Chotwiwatthanakun
Rapeepun Vanichviriyakit
Peter J. Hanna
Prasert Sobhon
Mahidol University
Deakin University
Keywords: Biochemistry, Genetics and Molecular Biology;Medicine
Issue Date: 1-Jun-2012
Citation: Cell and Tissue Research. Vol.348, No.3 (2012), 609-623
Abstract: Phosphorylated sperm proteins are crucial for sperm maturation and capacitation as a priori to their fertilization with eggs. In the freshwater prawn, Macrobrachium rosenbergii, a male reproduction-related protein (Mar-Mrr) was known to be expressed only in the spermatic ducts as a protein with putative phosphorylation and may be involved in sperm capacitation in this species.We investigated further the temporal and spatial expression of the Mar-Mrr gene using RT-PCR and in situ hybridization and the characteristics and fate of the protein using immunblotting and immunocytochemistry. The Mar-Mrr gene was first expressed in 4-week-old post larvae and the protein was produced in epithelial cells lining the spermatic ducts, at the highest level in the proximal region and decreased in the middle and distal parts. The native protein had a MW of 17 kDa and a high degree of serine/threonine phosphorylation. It was transferred from the epithelial cells to become a major protein at the anterior region of the sperm.We suggest that it is involved in sperm capacitation and fertilization in this open thelycal species and this is being investigated. © Springer-Verlag 2012.
ISSN: 14320878
Appears in Collections:Scopus 2011-2015

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