Simple jQuery Dropdowns
Please use this identifier to cite or link to this item:
Title: The toxin/immunity network of Burkholderia pseudomallei contact-dependent growth inhibition (CDI) systems
Authors: Kiel Nikolakakis
Saba Amber
J. Scott Wilbur
Elie J. Diner
Stephanie K. Aoki
Stephen J. Poole
Apichai Tuanyok
Paul S. Keim
Sharon Peacock
Christopher S. Hayes
David A. Low
University of California, Santa Barbara
Northern Arizona University
Translational Genomics Research Institute
Mahidol University
University of Arizona
University of California, Berkeley
ETH Zurich
University of Cambridge
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 1-May-2012
Citation: Molecular Microbiology. Vol.84, No.3 (2012), 516-529
Abstract: Burkholderia pseudomallei is a category B pathogen and the causative agent of melioidosis - a serious infectious disease that is typically acquired directly from environmental reservoirs. Nearly all B. pseudomallei strains sequenced to date ( > 85 isolates) contain gene clusters that are related to the contact-dependent growth inhibition (CDI) systems of γ-proteobacteria. CDI systems from Escherichia coli and Dickeya dadantii play significant roles in bacterial competition, suggesting these systems may also contribute to the competitive fitness of B. pseudomallei. Here, we identify 10 distinct CDI systems in B. pseudomallei based on polymorphisms within the cdiA-CT/cdiI coding regions, which are predicted to encode CdiA-CT/CdiI toxin/immunity protein pairs. Biochemical analysis of three B. pseudomallei CdiA-CTs revealed that each protein possesses a distinct tRNase activity capable of inhibiting cell growth. These toxin activities are blocked by cognate CdiI immunity proteins, which specifically bind the CdiA-CT and protect cells from growth inhibition. Using Burkholderia thailandensis E264 as a model, we show that a CDI system from B. pseudomallei 1026b mediates CDI and is capable of delivering CdiA-CT toxins derived from other B. pseudomallei strains. These results demonstrate that Burkholderia species contain functional CDI systems, which may confer a competitive advantage to these bacteria. © 2012 Blackwell Publishing Ltd.
ISSN: 13652958
Appears in Collections:Scopus 2011-2015

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.