Simple jQuery Dropdowns
Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorKiel Nikolakakisen_US
dc.contributor.authorSaba Amberen_US
dc.contributor.authorJ. Scott Wilburen_US
dc.contributor.authorElie J. Dineren_US
dc.contributor.authorStephanie K. Aokien_US
dc.contributor.authorStephen J. Pooleen_US
dc.contributor.authorApichai Tuanyoken_US
dc.contributor.authorPaul S. Keimen_US
dc.contributor.authorSharon Peacocken_US
dc.contributor.authorChristopher S. Hayesen_US
dc.contributor.authorDavid A. Lowen_US
dc.contributor.otherUniversity of California, Santa Barbaraen_US
dc.contributor.otherNorthern Arizona Universityen_US
dc.contributor.otherTranslational Genomics Research Instituteen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherUniversity of Arizonaen_US
dc.contributor.otherUniversity of California, Berkeleyen_US
dc.contributor.otherETH Zurichen_US
dc.contributor.otherUniversity of Cambridgeen_US
dc.identifier.citationMolecular Microbiology. Vol.84, No.3 (2012), 516-529en_US
dc.description.abstractBurkholderia pseudomallei is a category B pathogen and the causative agent of melioidosis - a serious infectious disease that is typically acquired directly from environmental reservoirs. Nearly all B. pseudomallei strains sequenced to date ( > 85 isolates) contain gene clusters that are related to the contact-dependent growth inhibition (CDI) systems of γ-proteobacteria. CDI systems from Escherichia coli and Dickeya dadantii play significant roles in bacterial competition, suggesting these systems may also contribute to the competitive fitness of B. pseudomallei. Here, we identify 10 distinct CDI systems in B. pseudomallei based on polymorphisms within the cdiA-CT/cdiI coding regions, which are predicted to encode CdiA-CT/CdiI toxin/immunity protein pairs. Biochemical analysis of three B. pseudomallei CdiA-CTs revealed that each protein possesses a distinct tRNase activity capable of inhibiting cell growth. These toxin activities are blocked by cognate CdiI immunity proteins, which specifically bind the CdiA-CT and protect cells from growth inhibition. Using Burkholderia thailandensis E264 as a model, we show that a CDI system from B. pseudomallei 1026b mediates CDI and is capable of delivering CdiA-CT toxins derived from other B. pseudomallei strains. These results demonstrate that Burkholderia species contain functional CDI systems, which may confer a competitive advantage to these bacteria. © 2012 Blackwell Publishing Ltd.en_US
dc.rightsMahidol Universityen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleThe toxin/immunity network of Burkholderia pseudomallei contact-dependent growth inhibition (CDI) systemsen_US
Appears in Collections:Scopus 2011-2015

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.