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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/13750
Title: Comparison of seven commercial antigen and antibody enzyme-linked immunosorbent assays for detection of acute dengue infection
Authors: Stuart D. Blacksell
Richard G. Jarman
Robert V. Gibbons
Ampai Tanganuchitcharnchai
Mammen P. Mammen
Ananda Nisalak
Siripen Kalayanarooj
Mark S. Bailey
Ranjan Premaratna
H. Janaka De Silva
Nicholas P J Day
David G. Lalloog
Mahidol University
University of Oxford
Armed Forces Research Institute of Medical Sciences, Thailand
Queen Sirikit National Institute of Child Health
Royal Centre for Defence Medicine
University of Kelaniya
Liverpool School of Tropical Medicine
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology;Medicine
Issue Date: 1-May-2012
Citation: Clinical and Vaccine Immunology. Vol.19, No.5 (2012), 804-810
Abstract: Seven commercial assays were evaluated to determine their suitability for the diagnosis of acute dengue infection: (i) the Panbio dengue virus Pan-E NS1 early enzyme-linked immunosorbent assay (ELISA), second generation (Alere, Australia); (ii) the Panbio dengue virus IgM capture ELISA (Alere, Australia); (iii) the Panbio dengue virus IgG capture ELISA (Alere, Australia); (iv) the Standard Diagnostics dengue virus NS1 antigen ELISA (Standard Diagnostics, South Korea); (v) the Standard Diagnostics dengue virus IgM ELISA (Standard Diagnostics, South Korea); (vi) the Standard Diagnostics dengue virus IgG ELISA (Standard Diagnostics, South Korea); and (vii) the Platelia NS1 antigen ELISA (Bio-Rad, France). Samples from 239 Thai patients confirmed to be dengue virus positive and 98 Sri Lankan patients negative for dengue virus infection were tested. The sensitivities and specificities of the NS1 antigen ELISAs ranged from 45 to 57% and 93 to 100% and those of the IgM antibody ELISAs ranged from 85 to 89% and 88 to 100%, respectively. Combining the NS1 antigen and IgM antibody results from the Standard Diagnostics ELISAs gave the best compromise between sensitivity and specificity (87 and 96%, respectively), as well as providing the best sensitivity for patients presenting at different times after fever onset. The Panbio IgG capture ELISA correctly classified 67% of secondary dengue infection cases. This study provides strong evidence of the value of combining dengue virus antigen- and antibody-based test results in the ELISA format for the diagnosis of acute dengue infection. Copyright © 2012, American Society for Microbiology.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84860336516&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/13750
ISSN: 1556679X
15566811
Appears in Collections:Scopus 2011-2015

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