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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/13781
Title: Influence of immobilized biomolecules on magnetic bead plug formation and retention in capillary electrophoresis
Authors: Rachel L. Henken
Rattikan Chantiwas
S. Douglass Gilman
Louisiana State University
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Chemistry
Issue Date: 1-Mar-2012
Citation: Electrophoresis. Vol.33, No.5 (2012), 827-833
Abstract: Significant changes in the formation and retention of magnetic bead plugs in a capillary during electrophoresis were studied, and it was demonstrated that these effects were due to the type of biological molecule immobilized on the surface of these beads. Three biological molecules, an antibody, an oligonucleotide, and alkaline phosphatase (AP), were attached to otherwise identical streptavidin-coated magnetic beads through biotin-avidin binding in order to isolate differences in bead immobilization in a magnetic field resulting from the type of biological molecule immobilized on the bead surface. AP was also attached to the magnetic beads using epoxy groups on the bead surfaces (instead of avidin-biotin binding) to study the impact of immobilization chemistry. The formation and retention of magnetic bead plugs were studied quantitatively using light scattering detection of magnetic particles eluting from the bead plugs and qualitatively using microscopy. Both the types of biomolecule immobilized on the magnetic bead surface and the chemistry used to link the biomolecule to the magnetic bead impacted the formation and retention of the bead plugs. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84860241722&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/13781
ISSN: 15222683
01730835
Appears in Collections:Scopus 2011-2015

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