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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/13785
Title: The role of the oligosaccharide binding cleft of rice BGlu1 in hydrolysis of cellooligosaccharides and in their synthesis by rice BGlu1 glycosynthase
Authors: Salila Pengthaisong
Stephen G. Withers
Buabarn Kuaprasert
Jisnuson Svasti
James R. Ketudat Cairns
Suranaree University of Technology
The University of British Columbia
Synchrotron Light Research Institute (Public Organization)
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Mar-2012
Citation: Protein Science. Vol.21, No.3 (2012), 362-372
Abstract: Rice BGlu1 b-glucosidase nucleophile mutant E386G is a glycosynthase that can synthesize p-nitrophenyl (pNP)-cellooligosaccharides of up to 11 residues. The X-ray crystal structures of the E386G glycosynthase with and without a-glucosyl fluoride were solved and the a-glucosyl fluoride complex was found to contain an ordered water molecule near the position of the nucleophile of the BGlu1 native structure, which is likely to stabilize the departing fluoride. The structures of E386G glycosynthase in complexes with cellotetraose and cellopentaose confirmed that the side chains of N245, S334, and Y341 interact with glucosyl residues in cellooligosaccharide binding subsites 12, 13, and 14. Mutants in which these residues were replaced in BGlu1 b-glucosidase hydrolyzed cellotetraose and cellopentaose with kcat/Km values similar to those of the wild type enzyme. However, the Y341A, Y341L, and N245V mutants of the E386G glycosynthase synthesize shorter pNPcellooligosaccharides than do the E386G glycosynthase and its S334A mutant, suggesting that Y341 and N245 play important roles in the synthesis of long oligosaccharides. X-ray structural studies revealed that cellotetraose binds to the Y341A mutant of the glycosynthase in a very different, alternative mode not seen in complexes with the E386G glycosynthase, possibly explaining the similar hydrolysis, but poorer synthesis of longer oligosaccharides by Y341 mutants. Published by Wiley-Blackwell. © 2012 The Protein Society.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84857405167&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/13785
ISSN: 1469896X
09618368
Appears in Collections:Scopus 2011-2015

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